Kirchgesser and Dahlmann: Colorimetric assay of acid nucleoside triphosphatase
نویسندگان
چکیده
A photometric method for the determination of the acid nucleoside triphosphatase (EC 3.6.1.—) is described, in which inorganic phosphate is liberated from ATP or other nucleoside triphosphates. Colorimetric determination of liberated phosphate is based on the formation of a green complex of phosphomolybdate and malachite green hydrochloride. Optimal test conditions were evaluated as well as the sample preparation. The enzyme activities measured in 100 normal human sera are in the range of 0.5 to 9.0 U/l with an average of 4.0 U/l for men and 3.8 U/l for women.
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